Overview | Coriolus Versicolor polysaccharide is a macromolecular proteoglycan oral agent extracted from the fermentation mycelia of Coriolus Versicolor. It can increase the level of interferon and IL-1 in vivo, activate macrophages in vivo, enhance cytotoxic activity, increase the uptake of thymidine by lymphocytes, and improve cellular immune function, reduce the side effects of anticancer chemotherapy or radiotherapy, clinical can be used for chronic hepatitis, liver cancer, lung cancer and gastrointestinal cancer and other diseases, as an adjuvant therapy. |
Structure of Coriolus versicolor polysaccharide | different strains and culture conditions, the molecular weight and structure of Coriolus versicolor polysaccharide may be different. Polysaccharide can be produced by Coriolus versicolor mycelium in vivo and in vitro. The extracellular polysaccharide extracted from Coriolus versicolor fermentation broth is glucan without peptide, containing β( 1 → 3) main chain and β( 1 → 3) and β( 1 → 6) branched chain, D-glucose content of 99. 2%, Coriolus versicolor fermentation broth also contains peptides, alkaloids, phenols, plant sterols, diterpenoids and triterpenoids, 16 kinds of amino acids, VB1, VB2, VB6 and copper, iron, potassium, zinc and other 10 kinds of trace elements needed by the human body; The intracellular polysaccharide extracted from the mycelium is the glycopeptide containing protein, and the main components are β( 1 → 4) and β( 1 → 3). Or beta (1 → 4), Beta (1 → 6) as the main chain, side chain with beta (1 → 3) or beta (1 → 6) combined with the branch, polysaccharide content was 30% ~ 60%, protein content was 10% ~ 30%, the main monosaccharide composition was glucose, followed by mannose, a small amount of galactose, xylose and fucose. |
extracted from Coriolus versicolor | . It belongs to Basidiomycetes (basiomyecta), laminaria (hymynems), non-pleurophylla (aphilophores), Polyporaceae, Coriolus or. On the piles, overlaid Wood and branches of a variety of broad-leaved trees (willow, poplar, Birch, plum, peach, etc.), occasionally on the trunk of pine trees, distributed all over the world, in the northern hemisphere of the original forest and the forest is more common; China's Coriolus versicolor is mainly distributed in the Northeast forest. The fruiting body of Coriolus versicolor is fan-shaped, semicircular or shell-shaped, without handle. A constant number of overlays generate shingle-like or rosette-like, 1 ~ 10cm in diameter, 1 ~ 4mm in thickness, with dense gray, brown, blue, purple, black and other color fluff on the surface, and the formation of a variety of colors of narrow concentric layer with thin edges, the whole edge or wave-like, pipe mouth face gray-brown, yellow-brown or light yellow, round or polygonal pipe mouth, part of the pipe mouth crack. Coriolus versicolor is rich in protein, fat, polysaccharide, polysaccharide peptide, glucan, lignin, amino acids and a variety of inorganic salts, as well as protease, peroxidase, amylase, laccase and enzyme. Coriolus versicolor polysaccharide is the main active ingredient in Coriolus versicolor extract. Figure 1: Coriolus versicolor
pharmacological action | 1. Anti-tumor effect Coriolus versicolor polysaccharide is an immunomodulator and immunopotentiator, which plays an anti-tumor effect by regulating and enhancing the body's immune function, especially enhancing the function of T lymphocytes. Coriolus versicolor polysaccharide was effective against AH -13 cells, Ketian's sarcoma cells, AH -130 cells and Ali's ascites cancer cells in vitro; Animal in vivo test against S-180, Ali's ascites cancer, leukemia-P388, MC-sarcoma, adenocarcinoma -755, ascites liver cancer-AH -13, AH -7974, AH -66F and keitian sarcoma were effective. The inhibitory rate of Coriolus versicolor polysaccharide on B16 melanoma was 84.4%. The inhibitory effect on the proliferation of human T acute lymphoblastic leukemia cell line Jurkat was slightly weak, with an inhibition rate of 70%, while the inhibitory effect on the proliferation of human colorectal adenocarcinoma cell line LS-180 was 58%; there was no significant inhibitory effect on human breast cancer cell MCF7 in the experimental concentration. The polysaccharide from Coriolus versicolor had obvious inhibitory effect on human chronic myelogenous leukemia cell line K562 and human colorectal adenocarcinoma cell line SW620, and the inhibitory rate was over 200 at the concentration of 60% μg/mL; 500g/mL, the inhibition rate of 90%. 2. To enhance the immune function of Coriolus versicolor polysaccharide can significantly increase 60Co irradiation induced immune function of mice serum lysozyme content and spleen index. The polysaccharide of Coriolus versicolor had obvious recovery effect on the reduction of white blood cells induced by cyclophosphamide, and could significantly increase the phagocytic index and phagocytic coefficient. Coriolus versicolor polysaccharide can significantly increase the levels of interleukin 2, lymphocyte toxin and interferon gamma in mouse spleen cells, and can significantly enhance the ability of peritoneal macrophages to secrete tumor necrosis factor and interleukin 1. Coriolus versicolor glycopeptide can promote the highly efficient expression of interleukin 6 in human peripheral blood induced by plant agglutinin, which is more obvious in induced culture for 48h. At the same time, the transcription of interleukin 6mRNA also shows obvious promoting effect, this may be the mechanism of its high expression of interleukin 6. Coriolus versicolor polysaccharide can improve the normal mice and cyclophosphamide-induced immunosuppression in mice lymphocyte transformation rate, sheep erythrocyte hemagglutination antibody titer, spleen cells produce hemolytic antibody ability and NK cells and macrophages anti-tumor activity. Coriolus versicolor polysaccharide can increase the activity of colony stimulating factor and the content of colony stimulating factor mRNA in mice. Coriolus versicolor polysaccharide can enhance nitric oxide induced macrophage apoptosis, induce nitric oxide synthase gene expression, and can enhance the induction of interferon gamma and lipopolysaccharide. After co-culture of mouse peritoneal macrophages treated with Coriolus versicolor polysaccharide and O-LDL for 24h, the cell morphology had no significant change, the production of tumor necrosis factor and nitric oxide was higher than that of the control group. Coriolus versicolor polysaccharide on total T cells and helper or inducer T cells have expansion effect. The polysaccharide of Coriolus versicolor can make the endotoxin clearance coefficient k more than 2 times of the control group, suggesting that the polysaccharide of Coriolus versicolor can significantly enhance the function of the body's reticuloendothelial system, and has a positive significance for the prevention and treatment of liver disease. 3. Antioxidant effect intraperitoneal injection to mice 1. 5% Coriolus versicolor polysaccharide 0 .2mL/only, continuous 4d, can improve the mouse body tissue selenium glutathione peroxidase activity, improve the body defense reactive oxygen species damage ability, its enhancement effect on the enzymatic scavenging mechanism of reactive oxygen species is closely related to its anti-tumor immune regulation effect, which is of great significance. It was found that the effect of polysaccharide on the expression of selenium glutathione peroxidase and non selenium glutathione peroxidase occurred at the transcription level, And the synthesis of a new protein may be involved in the induction process. Coriolus versicolor polysaccharide has obvious protective effect on the damage caused by oxidized low density lipoprotein. Small dose of tert-butyl hydroperoxide had no significant effect on the content of selenium glutathione peroxidase mRNA in peritoneal cavity of mice treated with Coriolus versicolor polysaccharide in vitro, but could induce selenium glutathione peroxidase mRNA in normal mouse peritoneal macrophages. Coriolus versicolor polysaccharide can enhance the resistance of macrophage respiratory burst function to tert-butyl hydroperoxide damage. 4. Effects on fecundity and life span of Coriolus versicolor polysaccharide can significantly increase the feeding frequency of Drosophila melanogaster, the number of mating times and the number of young flies in the next generation, but also can prolong the average life span of male flies, half time of death and the highest life span of female flies. 5. Effect on endocrine system (1) hypoglycemic effect to mice gavage Coriolus versicolor polysaccharide 0 .5g/kg, continuous 6d, can significantly reduce the rat tail vein injection of four oxygen caused by high blood sugar levels, and the four oxygen caused by high blood sugar has a preventive effect. (2) lipid-lowering effect mouse peritoneal macrophages were incubated with cell culture medium containing Coriolus versicolor polysaccharide 100 μg/mL at 37 ℃ for 48h, it was found that Coriolus versicolor polysaccharide could increase macrophage surface binding marker (125I) low density lipoprotein, and promote the internalization and degradation of the latter. The percentage of aortic plaque area in experimental atherosclerosis rabbits was significantly smaller than that in the control group (P6. The role of the digestive system (1) to protect the liver to mice gavage Coriolus versicolor polysaccharide 1g/kg, in 4H, 10h, 26h respectively 0. 13% CCl4 1 times, can significantly reduce the serum alanine aminotransferase in mice. Intragastric administration of 250mg/kg polysaccharide from Coriolus versicolor to mice for 7 consecutive days significantly reduced serum alanine aminotransferase and liver homogenate alanine aminotransferase in mice with subacute hepatitis. Intragastric administration of 300mg/kg polysaccharide from Coriolus versicolor to mice for 14 days can significantly prolong the survival period of mice with ascites type liver cancer, significantly reduce the number of cancer cells in ascites, and significantly inhibit the mitotic index of cancer cells. (2) anti-ulcer effect intragastric administration of Coriolus versicolor glycopeptide 4 .5g/kg, continuous 2d, can significantly inhibit the stress type, pyloric ligation type, acetic acid type and indomethacin type of experimental gastric ulcer, and can significantly reduce the total acidity of gastric juice of pyloric ligation type ulcer, however, there was no significant effect on the secretion of gastric juice, pepsin activity, free acidity and PGE2 content. 7. The role of the central nervous system (1) to improve learning and memory function to mice and rats gavage polysaccharide 500mg/kg, continuous 6d, can significantly improve the mice and rats in the shuttle box method, one-time training method and Y-maze method in the learning and memory function; Scopolamine caused by chemical memory disorder has a good protective effect, this may be related to the fact that the active ingredients contained therein provide sufficient energy for brain activity and synthesis of neurotransmitters. In addition, Coriolus versicolor polysaccharide on learning and memory impairment caused by Morris water maze also has obvious improvement effect. (2) anti-injury and anti-inflammatory effects: intraperitoneal injection of 200mg/kg, 100mg/kg and 50mg/kg of Coriolus versicolor polysaccharide can significantly inhibit the ear swelling of mice induced by croton oil, and showed a dose-dependent relationship; Coriolus versicolor polysaccharide can also significantly inhibit endotoxin-induced capillary permeability in mice. The polysaccharide of Coriolus versicolor has a reliable inhibitory effect on the diffuse emphysema and chronic lung inflammation induced by cigarette smoke in mice. In the Death experiment of mice caused by barium chloride, Coriolus versicolor polysaccharide can significantly prolong the survival time of mice. Coriolus versicolor polysaccharide can also significantly prolong the survival time of mice under Hypoxemia caused by sodium nitrite. It can significantly inhibit the pulmonary edema caused by epinephrine and the metastasis of peripheral blood leukocytes in the low white blood cell. (3) analgesic effect to mice gavage Coriolus versicolor glycopeptide 0 .5g/kg, 1g/kg, continuous 7d, can significantly improve the pain threshold of mice; And large dose (5~8 g/kg) not only can not improve the analgesic effect, on the contrary, the analgesic effect decreased. Coriolus versicolor polysaccharide has obvious analgesic effect on inflammatory pain caused by formaldehyde solution, carrageenan (acute) and adjuvant arthritis (chronic), and has obvious dose-effect relationship, the analgesic effect subsided about 2h after drug administration. Compared with pethidine, the analgesic effect was weaker and the duration was shorter. Its mechanism of action is that the polysaccharide can activate IL-2, which enters the brain and acts on the L -2 receptor of the hypothalamic matrix nerve to play an analgesic effect. Other effects of Coriolus versicolor polysaccharide can significantly protect influenza Virus intravenous infection caused by death and liver damage. Intraperitoneal injection of Coriolus versicolor polysaccharide can significantly enhance the non-specific resistance of mice to Staphylococcus aureus, Escherichia coli, Shigella sonnei and Pseudomonas aeruginosa infection. Coriolus versicolor polysaccharide on cyclophosphamide-induced micronucleus rate increased significantly against the role of cigarette smoke toxicity caused by chromosome damage of bone marrow micronucleus rate was significantly reduced. Coriolus versicolor polysaccharide can inhibit the proliferation of mammary epithelial cells induced by 7,12-dimethylbenzo anthracene (DMBA), especially the moderate and severe atypical hyperplasia. Coriolus versicolor polysaccharide also has the clinical curative effect of blocking the canceration of oral leukoplakia. |
Clinical application | is mainly used for gastric cancer, esophageal cancer, colon cancer, rectal cancer and other gastrointestinal cancer, can improve symptoms, make patients appetite, weight gain, pain reduction, and see Chest, ascites reduction, but also the role of tumor reduction. It has certain preventive effect on recurrence of digestive tract tumor, lung cancer, uterine cancer and breast cancer after operation. Coriolus versicolor polysaccharide K combined with mitomycin, cyclophosphamide, cytarabine, fluorouracil and other chemotherapy drugs, can enhance the anti-tumor effect, and small dose of local radiation in the treatment of cervical cancer, the effect is the same as that of high-dose radiotherapy. (2015-09-24) |
trait | Brown or brownish powder, slightly odorless and tasteless. Melting point 120 °c. Soluble in water, almost insoluble in methanol, chloroform, benzene, cyclohexane, etc. |
adverse reactions and precautions | Nausea, Vomit, Diarrhea and other gastrointestinal reactions are the main adverse reactions, the incidence rate was 1.01%. |
Use | This product is used as an anticancer drug, and its anticancer effect may be related to the improvement of immune function, simple or with other therapies in the treatment of digestive tract cancer, lung cancer, malignant lymphoma, etc. can improve the subjective symptoms, promote appetite, relieve pain, reduce the tumor, no adverse reactions. |